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GrainGenes Marker Report: WMC27

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Probe	WMC27
Locus	Xwmc27.2 [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2f9 Donor: Wang, Utah Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 352 bases located on 2D, 398 bases located on 2B (GT)25 153 to 202.
Type	PCR SSR
PCR primers	Forward AATAgAAAcAggTcAccATccg at 37 length 22 bases, Tm 62 Reverse TAgAgcTggAgTAgggccAAAg at 404 length 22 bases, Tm 64
SSR size	389 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC27
Source Species	Triticum aestivum