GrainGenes Marker Report: WMC274
Locus
wmc274
Type
Microsatellite
Map
Durum Wheat, NSGC Lr 3B 211.1 Physical-3B 174.82 Wheat, Yr genes and QTL 3B 99.6 Wheat_Yr_2021-3B 212.64
[ Show Nearby Loci ]
Map Data
3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat, Yr Update, 2021
Probe
WMC274
Possible Orthologs
Xwmc274-3B wmc274-3B
Probe
WMC274
Locus
Xwmc274-3B wmc274 wmc274-3B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 3h8 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 135 bases located on 3B No trimming done to sequence. (GAAAA)9 microsatellite 145-187.
Type
PCR SSR
PCR primers
Forward AAgcAAgcAgcAAAAcTATcAA at 92 length 22 bases, Tm 61
Reverse gAATgAATgAATgAATcgAggc at 209 length 22 bases, Tm 62
SSR size
139 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC274
Source Species
Triticum aestivum
GrainGenes Marker Report: WMC274
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