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GrainGenes Marker Report: WMC277

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Probe	WMC277
Locus	Xwmc277.3
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3a10 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 7B No trimming done to sequence. (CA)18 248 to 283.
Type	PCR SSR
PCR primers	Forward cATAgTgTgAccAAcTAcgAAc at 187 length 22 bases, Tm 57 Reverse gTgTgcTAAccTcTATAgTgTg at 280 length 22 bases, Tm 54
SSR size	115 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC277
Source Species	Triticum aestivum