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GrainGenes Marker Report: WMC278

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Probe	WMC278
Locus	Xwmc278-1A Xwmc278 wmc278
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3f10 Donor: Keller, Zurich No trimming done to sequence. (GT)14 200 to 227. Bases 200-300 (ie. 3' side of microsatellite) match part of 11b12
Type	PCR SSR
PCR primers	Forward AAAcgATAgTAAAATTAccTcggAT at 117 length 25 bases, Tm 59 Reverse TcAAAAAATAgcAAcTTgAAgAcAT at 257 length 25 bases, Tm 60
SSR size	165 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC278
Source Species	Triticum aestivum