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GrainGenes Marker Report: WMC289

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Probe	WMC289
Locus	Xwmc289 [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13d2 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 164 bases located on 5D, 198 bases located on 5B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)13 221 to 246, (CT)10 178 to 197.
Type	PCR SSR
PCR primers	Forward cATATgcATgcTATgcTggcTA at 146 length 22 bases, Tm 63 Reverse AgccTTTcAAATccATccAcTg at 324 length 22 bases, Tm 64
SSR size	200 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC289
Source Species	Triticum aestivum