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GrainGenes Marker Report: WMC310

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Probe	WMC310
Locus	Xwmc310 Xwmc310-4B wmc310
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14f1 Donor: Hjortsholm, Sejet Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 191 bases located on 4B Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)10 329 to 348 plus (GT)4.
Type	PCR SSR
PCR primers	Forward TgTgAggcTgggAggAAAAgAg at 205 length 22 bases, Tm 67 Reverse gcTAggTTgTgTcccAcAATgc at 376 length 22 bases, Tm 66
SSR size	193 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.
Sequence	WMC310
Source Species	Triticum aestivum