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GrainGenes Marker Report: WMC320

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Locus	wmc320
Type	Microsatellite
Map	Physical-3B 62.85
	[ Show Nearby Loci ]
Map Data	3B Physical
Probe	WMC320
Possible Orthologs	Xwmc320
Probe	WMC320
Locus	Xwmc320 wmc320
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 14a6 Donor: Hjortsholm, Sejet Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)12 24 to 47.
Type	PCR SSR
PCR primers	Forward cATgcTTTcAAgAccTAcgAcA at 4 length 22 bases, Tm 62 Reverse gcAAAcTTgcTcTgcTTgAcTc at 78 length 22 bases, Tm 63
SSR size	96 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide.
Sequence	WMC320
Source Species	Triticum aestivum