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GrainGenes Marker Report: WMC327

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Probe
WMC327
Locus
Xwmc327-5A
Xwmc327
wmc327
wmc327-5A
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 17e9
Donor: Khairallah, Mexico
Trimmed to insert at both ends. Bases 1 to 15 show homology to 1H3 bases 116 to 130 (15 in longest run, maxScore = 24), zone is 1-40. Primers are outside of this region. (GT)25 150 to 199.
Type
PCR
SSR
PCR primers
Forward TgcggTAcAggcAAggcT at 75 length 18 bases, Tm 65
Reverse TAgAAcgcccTcgTcggA at 240 length 18 bases, Tm 65
SSR size
183 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide.
Sequence
WMC327
Source Species
Triticum aestivum