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GrainGenes Marker Report: WMC332

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Probe	WMC332
Locus	Xwmc332-2B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 17b12 Donor: Khairallah, Mexico Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 172 bases located on 2B Trimed to insert at both ends. (CT)12 136 to 159.
Type	PCR SSR
PCR primers	Forward cATTTAcAAAgcgcATgAAgcc at 51 length 22 bases, Tm 65 Reverse gAAAAcTTTgggAAcAAgAgcA at 198 length 22 bases, Tm 62
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC332
Source Species	Triticum aestivum