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GrainGenes Marker Report: WMC338

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Probe	WMC338
Locus	Xwmc338
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12g4 Donor: Weibull, Svaloff Weibull Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)22 185 to 228.
Type	PCR SSR
PCR primers	Forward AgATccgTATgATATgcTTgcg at 148 length 22 bases, Tm 63 Reverse cTTcATcAgccTccATgAcAcA at 262 length 22 bases, Tm 65
SSR size	136 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC338
Source Species	Triticum aestivum