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GrainGenes Marker Report: WMC339

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Probe	WMC339
Locus	Xwmc339-1D wmc339
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12b5 Donor: Weibull, Svaloff Weibull Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 208 bases located on 6B Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)8 118 to 133.
Type	PCR SSR
PCR primers	Forward ccgcTcgccTTcTTccAg at 29 length 18 bases, Tm 66 Reverse TccggAAcATgccgATAc at 215 length 18 bases, Tm 64
SSR size	204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC339
Source Species	Triticum aestivum