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GrainGenes Marker Report: WMC341

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Probe	WMC341
Locus	Xwmc341
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12f5 Donor: Weibull, Svaloff Weibull Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)24 55 to 102.
Type	PCR SSR
PCR primers	Forward AcATgggcTAcATgAgAgAAgA at 21 length 22 bases, Tm 61 Reverse AgAgTggcTcccTTTTcAccTA at 119 length 22 bases, Tm 63
SSR size	120 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC341
Source Species	Triticum aestivum