Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: WMC366

[Submit comment/correction]

Locus	wmc366
Type	Microsatellite
Map	Physical-3B 59.6 Wheat, Yr genes and QTL 3B 40.3 Wheat_Yr_2021-3B 86.04
	[ Show Nearby Loci ]
Map Data	3B Physical Wheat, Yr genes and QTL Wheat, Yr Update, 2021
Probe	WMC366
Possible Orthologs	Xwmc366 Xwmc366-3B
Probe	WMC366
Locus	Xwmc366 Xwmc366-3B wmc366
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d8.1 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 112 bases located on 3B Trimmed to insert at both ends. Multiple inserts (2) in this clone. This is the first insert. Bases 43 to 60 (complement) show homology to 9d4 bases 379 to 396 (18 in longest run, maxScore = 28). (CA)12 121 to 144, (CA)6 174 to 185.
Type	PCR SSR
PCR primers	Forward TAccTcTcTAcgATgAAgcc at 61 length 20 bases, Tm 56 Reverse TggAgTcTTAgTgTggTgTT at 151 length 20 bases, Tm 55
SSR size	110 bases expected, Chinese Spring
Amplification Conditions	51C anneal, smear.
Sequence	WMC366
Source Species	Triticum aestivum