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GrainGenes Marker Report: WMC367

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Probe	WMC367
Locus	Xwmc367 Xwmc367-1B wmc367
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 12d8.2 Donor: Mohler, Munich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 104 bases located on 1B Trimmed to insert at 5' and and to Rsa1 at 3' end. Multiple inserts (2) in this clone. This is the second insert. Small microsatellite (GCC)5 125 to 139.
Type	PCR SSR
PCR primers	Forward cTgAcgTTgATgggccAcTATT at 8 length 22 bases, Tm 65 Reverse gTggTggAAgAggAAggAgAgg at 140 length 20 bases, Tm 65
SSR size	154 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC367
Source Species	Triticum aestivum