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GrainGenes Marker Report: WMC397

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Probe	WMC397
Locus	Xwmc397-6B Xwmc0397.1-6B Xwmc397b wmc397
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10h12 Donor: Jacquemin, Gembloux Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 162 bases located on 3A, 364 bases located on 4B 5' end not located, trimmed to Rsa1 at 3' end. (CA)9 69 to 86, (GA)14 93 to 120.
Type	PCR SSR
PCR primers	Forward AgTcgTgcAccTccATTTTg at 46 length 20 bases, Tm 63 Reverse cATTggAcATcggAgAccTg at 186 length 20 bases, Tm 63
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC397
Source Species	Triticum aestivum