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GrainGenes Marker Report: WMC399

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Probe	WMC399
Locus	Xwmc399 Xwmc399-4D wmc399 wmc399-4D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22a8 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 174 bases located on 4D Trimmed to insert at both ends. (CA)13 260 to 285.
Type	PCR SSR
PCR primers	Forward cTTcAgAgATgTTTgATTAccT at 137 length 22 bases, Tm 55 Reverse ggTATTgcTAAcTgAATgATgT at 287 length 22 bases, Tm 55
SSR size	172 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC399
Source Species	Triticum aestivum