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GrainGenes Marker Report: WMC406

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Probe	WMC406
Locus	Xwmc406-1B Xwmc406 wmc406 wmc406-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22b12 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 227 bases located on 1B Trimmed to Ssp1 at both ends of insert. (CA)16 188 to 219.
Type	PCR SSR
PCR primers	Forward TATgAgggTcggATcAATAcAA at 116 length 22 bases, Tm 61 Reverse cgAgTTTAcTgcAAAcAAATgg at 311 length 22 bases, Tm 61
SSR size	217 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (though weak).
Sequence	WMC406
Source Species	Triticum aestivum