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GrainGenes Marker Report: WMC410

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Probe	WMC410
Locus	Xwmc410 wmc410 wmc410-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10g4 Donor: Gale, John Innes Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)26 69 to 120. Bases 62 to 137 show homology to 2d1 bases 1 to 76 (complement) (76 in longest run, maxScore = 58). True duplicate with 2d1, 10g4 has priority.
Type	PCR SSR
PCR primers	Forward ggAcTTgAAAggAAgcTTgTgA at 21 length 22 bases, Tm 63 Reverse cATggATggcATgcAgTgT at 118 length 19 bases, Tm 64
SSR size	116 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC410
Source Species	Triticum aestivum