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GrainGenes Marker Report: WMC417

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Probe	WMC417
Locus	Xwmc417-6A [ Show all 15 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21c1 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 113 bases located on 6B No trimming done to sequence at Agrogene, ends not located. (CA)26 56 to 107.
Type	PCR SSR
PCR primers	Forward gTTcTTTTAgTTgcgAcTgAgg at 21 length 22 bases, Tm 60 Reverse cgATgTATgccgTATgAATgTT at 142 length 22 bases, Tm 61
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide. (180-200 bases, plus other bands).
Sequence	WMC417
Source Species	Triticum aestivum