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GrainGenes Marker Report: WMC418

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Locus	wmc418
Type	Microsatellite
Map	Physical-3B 74.4 Wheat, Yr genes and QTL 3B 45.3 Wheat_Consensus_2014_Ug99_Rust_3B 80.11
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Map Data	3B Physical Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust
Probe	WMC418
Possible Orthologs	Xwmc418 Xwmc418-3B Xwmc418-3D wmc418-3B
Probe	WMC418
Locus	Xwmc418-3B Xwmc418 Xwmc418-3D wmc418 wmc418-3B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21d1 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 242 bases located on 3B, 264 bases located on 3B No trimming done to sequence at Agrogene, ends not located. Bases 56 to 555 show homology to 21g3 bases 58 to 557 (500 in longest run, maxScore = 423), duplicate, 21d1 has priority. (GT)13 295 to 320.
Type	PCR SSR
PCR primers	Forward AgAgcAgcAAgTTgTgTAgccA at 160 length 22 bases, Tm 65 Reverse TgAAgcTATTgccAgcAcgAg at 402 length 21 bases, Tm 65
SSR size	263 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (264-268 bases).
Sequence	WMC418
Source Species	Triticum aestivum