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GrainGenes Marker Report: WMC42

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Probe	WMC42
Locus	Xwmc42 Xwmc42-7D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6e1 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 235 bases located on 7D AGR53. Trimmed to insert . (GT)8 202 to 217. Polymorphism is in very large fragments (~400) , ~4 loci.
Type	PCR SSR
PCR primers	Forward gcccTTggTccTggggTgAgcc at 52 length 22 bases, Tm 76 Reverse gccTcATccAgAgAgccTgcgg at 259 length 22 bases, Tm 73
SSR size	229 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC42
Source Species	Triticum aestivum