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GrainGenes Marker Report: WMC420

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Probe	WMC420
Locus	Xwmc420-4A Xwmc420 Xwmc420-2A wmc420 wmc420-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21a2 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 126 bases located on 4A Trimmed to insert at 3' end, 5' end not located. (GT)22 62 to 105, (GT)4 108 to 115.
Type	PCR SSR
PCR primers	Forward ATcgTcAAcAAAATcTgAAgTg at 15 length 22 bases, Tm 59 Reverse TTAcTTTTgcTgAgAAAAcccT at 118 length 22 bases, Tm 59
SSR size	125 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (120-124 bases)
Sequence	WMC420
Source Species	Triticum aestivum