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GrainGenes Marker Report: WMC422

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Probe	WMC422
Locus	Xwmc422-7A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21h3 Donor: Langridge, CRC Australia Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 305 bases located on 7A No trimming done to sequence at Agrogene, ends not located. (CT)16 25 to 56.
Type	PCR SSR
PCR primers	Forward ggAcTAcTgAAcTggAgAgTgTg at 2 length 23 bases, Tm 60 Reverse gcATTAgAATTTggAgTTTggAg at 283 length 23 bases, Tm 61
SSR size	304 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (295-310 bases)
Sequence	WMC422
Source Species	Triticum aestivum