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GrainGenes Marker Report: WMC43

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Locus	wmc43
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 39 Physical-3B 37.78 Wheat, Yr genes and QTL 3B 31.9 Wheat_Consensus_2014_Ug99_Rust_3B 60.11 Wheat_Consensus_2014_Ug99_Rust_3D 16
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust
Probe	WMC43
Possible Orthologs	Xwmc43 [ Show all 12 ]
Probe	WMC43
Locus	Xwmc43-3B [ Show all 14 ]
Synonym	Synonym HAR35_25148 HAR35_49365
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6b2 Donor: Dubcovsky, Buenos Aires Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 323 bases located on 3B, 330 bases located on 3D AGR58. Trimmed to insert at 3' end. (GT)14 429 to 456.
Type	PCR SSR
PCR primers	Forward TAgcTcAAccAccAcccTAcTg at 194 length 22 bases, Tm 63 Reverse AcTTcAAcATccAAAcTgAccg at 493 length 22 bases, Tm 63
SSR size	321 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product;
Sequence	WMC43
Source Species	Triticum aestivum