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GrainGenes Marker Report: WMC441

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Probe	WMC441
Locus	Xwmc441-2B [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24d6 Donor: ITCF Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 159 bases located on 2B Trimmed to insert at both ends. Bases 144 to 164 show homology to 17h7 bases 131 to 151 (21 in longest run, maxScore = 38). (CA)10 53 to 72, (CG)9 73 to 90, (CT)9 35 to 52.
Type	PCR SSR
PCR primers	Forward TccAgTAgAgcAccTTTcATT at 5 length 21 bases, Tm 58 Reverse ATcAcgAAgATAAAcAAAcgg at 142 length 21 bases, Tm 58
SSR size	158 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (complex).
Sequence	WMC441
Source Species	Triticum aestivum