GrainGenes Marker Report: WMC446
Locus
wmc446
Type
Microsatellite
Map
Physical-3B 59.6 Wheat, Yr genes and QTL 3B 40 Wheat, Yr genes and QTL 4A 29.3 Wheat, Yr genes and QTL 5A 27
[ Show Nearby Loci ]
Map Data
3B Physical Wheat, Yr genes and QTL
Probe
WMC446
Possible Orthologs
Xwmc446
[ Show all 7 ]
Probe
WMC446
Locus
Xwmc446
[ Show all 8 ]
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 24e7 Donor: ITCF Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)9 33 to 50, (CA)3 52 to 57. Too difficult to make primer.
Type
PCR SSR
PCR primers
Forward ccAgcTAgTAcTcTATATcTAcATc at 9 length 25 bases, Tm 52
Reverse TATTTgAAcAAgAgTTATgTgg at 103 length 21 bases, Tm 54
SSR size
115 bases expected, Chinese Spring
Amplification Conditions
51C anneal, stuttery product.
Sequence
WMC446
Source Species
Triticum aestivum
GrainGenes Marker Report: WMC446
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|