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GrainGenes Marker Report: WMC450

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Probe	WMC450
Locus	Xwmc450-7D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2g1 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 294 bases located on 1A, 321 bases located on 7D, 439 bases located on 1A, 579 bases located on 1D Trimmed to insert at both ends. (GCC)5 100 to 114.
Type	PCR SSR
PCR primers	Forward gcAggAcAggAggTgAAgAAg at 79 length 21 bases, Tm 63 Reverse AggcgTTgcTgATgAcAcTAc at 208 length 21 bases, Tm 62
SSR size	150 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC450
Source Species	Triticum aestivum