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GrainGenes Marker Report: WMC455

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Probe	WMC455
Locus	Xwmc455 Xwmc455-2A wmc455
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2b4 Donor: IGS BioTech Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 115 bases located on 2A, 168 bases located on 2A Trimmed to Rsa1 at both ends of insert. Bases 232 to 262 show homology to 12c7 bases 55 to 85 (31 in longest run, maxScore = 24). (CA)6 219 to 230, (CA)15 233 to 262, (CA)5 265 to 274.
Type	PCR SSR
PCR primers	Forward gcgTcATTTccTcAAAcAcATc at 184 length 22 bases, Tm 63 Reverse AgAAggAgAAgTgccTcAccAA at 320 length 22 bases, Tm 64
SSR size	158 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC455
Source Species	Triticum aestivum