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GrainGenes Marker Report: WMC463

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Probe	WMC463
Locus	Xwmc463-7D SrTA10171 wmc463 Xwmc463
Reference	Olson EL et al. (2013) Introgression of stem rust resistance gene SrTA10187 and SrTA10171 from Aegilops tauschii to wheat Theoretical and Applied Genetics 126:2477-2484. Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stem Rust Resistance. SrTA10171 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21a8 Donor: Dupont Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 153 bases located on 7D Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)26 184 to 235.
Type	PCR SSR
PCR primers	Forward gATTgTATAgTcggTTAccccT at 121 length 22 bases, Tm 58 Reverse ATTAgTgcccTccATAATTgTg at 251 length 22 bases, Tm 60
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (115, 152-156 bases).
Sequence	WMC463
Source Species	Triticum aestivum