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GrainGenes Marker Report: WMC468

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Probe	WMC468
Locus	Xwmc468-4A Xwmc468 wmc468 wmc468-4A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 21g12 Donor: Dupont Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 156 bases located on 4A Trimmed to Ssp1 at 5' end and to insert at 3' end. (CT)21 145 to 186.
Type	PCR SSR
PCR primers	Forward AgcTgggTTAATAAcAgAggAT at 74 length 22 bases, Tm 58 Reverse cAcATAAcTgTccAcTccTTTc at 202 length 22 bases, Tm 58
SSR size	150 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (126-176 bases, complex).
Sequence	WMC468
Source Species	Triticum aestivum