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GrainGenes Marker Report: WMC471

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Locus	wmc471
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 4B 76.8 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Finch x Eltan Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust
Probe	WMC471
Possible Orthologs	Xwmc471 [ Show all 7 ]
Probe	WMC471
Locus	Xwmc471 [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e1 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 161 bases located on 4A Trimmed to insert at 5' end and to Ssp1 at 3' end. (GT)4 112 to 119, (GT)8 179 to 194, (GT)14 197 to 224.
Type	PCR SSR
PCR primers	Forward ggcAATAATAgTgcAAggAATg at 45 length 22 bases, Tm 60 Reverse gccgATAATgggcAATATAAgT at 235 length 22 bases, Tm 61
SSR size	212 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. 51C anneal, polymorphic on acrylamide.
Sequence	WMC471
Source Species	Triticum aestivum