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GrainGenes Marker Report: WMC474

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Probe	WMC474
Locus	Xwmc474-2B [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25e3 Donor: Jizeng, Beijing Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 2B Trimmed to insert at both ends. (GT)20 53 to 92.
Type	PCR SSR
PCR primers	Forward ATgcTATTAAAcTAgcATgTgTcg at 3 length 24 bases, Tm 60 Reverse AgTggAAAcATcATTccTggTA at 103 length 22 bases, Tm 60
SSR size	120 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product. 51C anneal, product.
Sequence	WMC474
Source Species	Triticum aestivum