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GrainGenes Marker Report: WMC49

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Probe	WMC49
Locus	Xwmc49-1B wmc49 wmc49-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6d4 Donor: Dubcovsky, Buenos Aires AGR76. Trimmed to insert at 5' end. (CT)19 141 to 178.
Type	PCR SSR
PCR primers	Forward cTcATgAgTATATcAccgcAcA at 82 length 22 bases, Tm 60 Reverse gAcgcgAAAcgAATATTcAAgT at 266 length 22 bases, Tm 62
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product;
Sequence	WMC49
Source Species	Triticum aestivum