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GrainGenes Marker Report: WMC493

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Probe	WMC493
Locus	Xgwm493-3B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25g9 Donor: Serasem Trimmed to insert at both ends. Bases 4 to 97 (complement) show homology to 3c4 bases 65 to 158 (94 in longest run, maxScore = 77). (TC)5 104 to 113, (CA)19 139 to 176, (CT)11 117 to 138.
Type	PCR SSR
PCR primers	Forward AcATAcAATgggATTccAgcAg at 57 length 22 bases, Tm 63 Reverse AAcAAAcggTgTTcATgcAAgT at 221 length 22 bases, Tm 63
SSR size	186 bases expected, Chinese Spring
Amplification Conditions	61C anneal, smear (120-200 bases)
Sequence	WMC493
Source Species	Triticum aestivum