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GrainGenes Marker Report: WMC494

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Probe	WMC494
Locus	Xwmc494-6B Xwmc494 wmc494 wmc494-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25a10 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 221 bases located on 5B, 5D, 6B, 7D Trimmed to insert at both ends. (GT)31 138 to 199.
Type	PCR SSR
PCR primers	Forward ggATcgAgTcTcAAgTcTAcAA at 13 length 22 bases, Tm 58 Reverse AgAAggAAcAAgcAAcATcATA at 209 length 22 bases, Tm 58
SSR size	218 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (160-220 bases).
Sequence	WMC494
Source Species	Triticum aestivum