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GrainGenes Marker Report: WMC50

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Probe	WMC50
Locus	Xwmc50-3A Xwmc50 Xwmc050 wmc50 wmc50-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6e5 Donor: Dubcovsky, Buenos Aires AGR85. Trimmed to insert at 3' end. Multiple inserts (2) joined 219-236 by linkers. Microsatelilite in first insert too close to end to make primers. First insert matches 7B1. Second insert (GT)10 and (GT)16 repeats 402 - 456. Primers are to second insert.
Type	PCR SSR
PCR primers	Forward cTgccgTcAggccAggcTcAcA at 349 length 22 bases, Tm 76 Reverse cAAccAgcTAgcTgccgccgAA at 472 length 22 bases, Tm 74
Amplification Conditions	61C anneal, product;
Sequence	WMC50
Source Species	Triticum aestivum