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GrainGenes Marker Report: WMC500

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Locus	wmc500
Type	microsatellite
Map	Durum Wheat, NSGC Lr 3B 27.1 [ Show all 10 ]
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	WMC500
Possible Orthologs	Xwmc500-1B.1 [ Show all 26 ]
Probe	WMC500
Locus	Xwmc500-3B [ Show all 28 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 25b12 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 155 bases located on 2B, 165 bases located on 7B, 180 bases located on 1B, 188 bases located on 3B, 204 bases located on 4A Trimmed to insert at both ends. (CA)13 38 to 63, (CA)10 66 to 85.
Type	PCR SSR
PCR primers	Forward ATAgcATgTTggAAcAgAgcAc at 15 length 22 bases, Tm 61 Reverse cTTAgATgcAAcTcTATgcggT at 178 length 22 bases, Tm 60
SSR size	185 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (polymorphism is superimposed on smear, 150-190 bases, complex).
Sequence	WMC500
Source Species	Triticum aestivum