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GrainGenes Marker Report: WMC506

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Probe	WMC506
Locus	Xwmc506-7D Xwmc506 wmc506
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20d9 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 221 bases located on 7D Trimmed to Ssp1 at 5' end and to insert at 3' end. (GA)5 85 to 94, (GA)24 105 to 152.
Type	PCR SSR
PCR primers	Forward cAcTTccTcAAcATgccAgA at 12 length 20 bases, Tm 62 Reverse cTTTcAATgTggAAggcgAc at 208 length 20 bases, Tm 62
SSR size	216 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC506
Source Species	Triticum aestivum