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GrainGenes Marker Report: WMC508

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Probe	WMC508
Locus	Xwmc508 Xwmc508-5B wmc508
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20e10 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 181 bases located on 5B Trimmed to insert at 3' end, 5' end not located. Bases 123 to 194 show homology to 8h11 bases 116 to 187 (72 in longest run, maxScore = 63), reverse primer is outside this region of homology, forward primer matches 8h11. (GA)6 83 to 94.
Type	PCR SSR
PCR primers	Forward AgcccTTgAgTTggTcTcATTT at 53 length 22 bases, Tm 63 Reverse gAgcAgAgcTccAcTcAcATTT at 210 length 22 bases, Tm 63
SSR size	179 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC508
Source Species	Triticum aestivum