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GrainGenes Marker Report: WMC513

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Probe	WMC513
Locus	Xwmc513 Xwmc513-4A wmc513
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24h9 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 226 bases located on 4A Trimmed to Ssp1 at 5' end and to insert at 3' end (3' linker corrupted ) . (CA)21 118 to 159.
Type	PCR SSR
PCR primers	Forward TgAATTgAATcTggTTgcgg at 58 length 20 bases, Tm 64 Reverse TggcAATTcAcAggcAcATA at 247 length 20 bases, Tm 63
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC513
Source Species	Triticum aestivum