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GrainGenes Marker Report: WMC52

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Probe	WMC52
Locus	Xwmc52-1B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6h5 Donor: Dubcovsky, Buenos Aires AGR88. Trimmed to Rsa1 fragment. (CA)20 420 to 459. relative 6g6. Penner maps to 1B, Jorge to 4D. We detect 2 loci.
Type	PCR SSR
PCR primers	Forward TccAATcAATcAgggAggAgTA at 322 length 22 bases, Tm 62 Reverse gAAcgcATcAAggcATgAAgTA at 492 length 22 bases, Tm 64
SSR size	192 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC52
Source Species	Triticum aestivum