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GrainGenes Marker Report: WMC527

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Locus	wmc527
Type	microsatellite
Map	Durum Wheat, NSGC Lr 3B 89.8 Physical-3B 63.68 Wheat, Yr genes and QTL 3A 49.4 Wheat, Yr genes and QTL 3B 45.2 Wheat_AB_2017_Carotenoid_QTL-3B 89.8
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	WMC527
Possible Orthologs	Xwmc527 [ Show all 9 ]
Probe	WMC527
Locus	Xwmc527 [ Show all 10 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24B10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 378 bases located on 3B Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)15 328 to 357, (GA)20 30 to 69.
Type	PCR SSR
PCR primers	Forward AcccAAgATTggTTgcAgAA at 4 length 20 bases, Tm 63 Reverse gcTAcAgAAAAccggAgccTAT at 366 length 22 bases, Tm 62
SSR size	386 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (360 bases).
Sequence	WMC527
Source Species	Triticum aestivum