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GrainGenes Marker Report: WMC533

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Locus	wmc533
Type	Microsatellite
Map	Physical-3B 64.32 Wheat, Yr genes and QTL 3B 40.6
	[ Show Nearby Loci ]
Map Data	3B Physical Wheat, Yr genes and QTL
Probe	WMC533
Possible Orthologs	Xwmc533 Xwmc533-3B Xwmc533-3D Xwmc533a wmc533-3B
Probe	WMC533
Locus	Xwmc533-3D [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26h1 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 134 bases located on 3B, 152 bases located on 3D Trimmed to insert at both ends. (CA)20 189 to 228.
Type	PCR SSR
PCR primers	Forward AATTggATcggcAgTTggAg at 131 length 20 bases, Tm 64 Reverse AgcAAgcAgAgcATTgcgTT at 258 length 20 bases, Tm 65
SSR size	147 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (140-156 bases).
Sequence	WMC533
Source Species	Triticum aestivum