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GrainGenes Marker Report: WMC539

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Probe	WMC539
Locus	Xwmc539 Xwmc539-6B wmc539 wmc539-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26a6 Donor: Madre, Secobra Trimmed to insert at both ends. (GT)64 75 to 202.
Type	PCR SSR
PCR primers	Forward gcAAgTAggAccTTAcAgTTcT at 27 length 22 bases, Tm 56 Reverse gTTATAAccTTTgTcccTTcAc at 204 length 22 bases, Tm 56
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (200 bases).
Sequence	WMC539
Source Species	Triticum aestivum