Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: WMC54

[Submit comment/correction]

Locus	wmc54
Type	Microsatellite
Map	Physical-3B 21.12
	[ Show Nearby Loci ]
Map Data	3B Physical
Probe	WMC54
Possible Orthologs	Xwmc54
Probe	WMC54
Locus	Xwmc54 wmc54
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1c7 Donor: Waugh, SCRI (GT)8 176 to 191, (GT)8 194 to 209. Bands tend to be faint.
Type	PCR SSR
PCR primers	Forward TATTgTgcAATcgcAgcATcTc at 141 length 22 bases, Tm 65 Reverse TgcgAcATTggcAAccAcTTcT at 261 length 22 bases, Tm 69
SSR size	142 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC54
Source Species	Triticum aestivum