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GrainGenes Marker Report: WMC544

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Probe	WMC544
Locus	Xwmc544-3B wmc544
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 10d5 Donor: Gale, John Innes No trimming done to sequence at Agrogene, ends not located. Bases 143 to 163 (complement) show homology to 17e12 bases 355 to 375 (21 in longest run, maxScore = 55). Forward and reverse primers no obvious match to 17e12. (CA)14 104 to 131, (CA)10 238 to 257.
Type	PCR SSR
PCR primers	Forward ccATTTgAggTTTggTcgcTAc at 65 length 22 bases, Tm 64 Reverse TATATgTgATTTgTcgTgcccc at 425 length 22 bases, Tm 63
SSR size	382 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC544
Source Species	Triticum aestivum