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GrainGenes Marker Report: WMC546

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Probe	WMC546
Locus	Xwmc546-4B [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26g7 Donor: Momont Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 172 to 202 (complement) show homology to 22d9 bases 22 to 52 (31 in longest run, maxScore = 58). Reverse has 4 mismatches to 22d9, forward primer no obvious match. (CA)9 95 to 112, (CA)8 115 to 130.
Type	PCR SSR
PCR primers	Forward cggcTAAAATcgTAcAcTAcAcA at 12 length 23 bases, Tm 61 Reverse cTcAcTTgcAcgATTTcccTAT at 141 length 22 bases, Tm 62
SSR size	151 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (110-170 bases, complex).
Sequence	WMC546
Source Species	Triticum aestivum