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GrainGenes Marker Report: WMC557

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Probe	WMC557
Locus	Xwmc557-7B Yr59 wmc557
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr59 MAS Wheat. Marker Assisted Selection in Wheat.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26b12 Donor: Momont Trimmed to insert at both ends. (GCC)6 218 to 235.
Type	PCR SSR
PCR primers	Forward ggTgcTTgTTcATAcgggcT at 44 length 20 bases, Tm 64 Reverse AggTccTcgATccgcTcAT at 323 length 19 bases, Tm 64
SSR size	298 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (140 bases, 280-320). Gives nulls for 140.
Sequence	WMC557
Source Gene	Yr59 (Triticum)
Source Species	Triticum aestivum