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GrainGenes Marker Report: WMC574

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Probe
WMC574
Locus
Xwmc574-2D
[ Show all 6 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 15f1.2
Donor: Aventis
Multiple inserts in this clone, this is the second insert. Trimmed to insert at 5' end and to Rsa1 at 3' end. (CT)24 148 to 195.
Type
PCR
SSR
PCR primers
Forward TccccTAcTggAAccAcgAc at 53 length 20 bases, Tm 64
Reverse ATccATcgAccgAcAAgAgc at 199 length 20 bases, Tm 64
SSR size
166 bases expected, Chinese Spring
Sequence
WMC574
Source Species
Triticum aestivum