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GrainGenes Marker Report: WMC58

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Probe	WMC58
Locus	Xwmc58-7A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1a8 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 7B, 233 bases located on 7A, 241 bases located on 7D (GCC)6 175 to 192
Type	PCR SSR
PCR primers	Forward ATcggcggTgAAgcTgATAAgT at 32 length 22 bases, Tm 66 Reverse TgAAAcAggggAATcAgAgggT at 247 length 22 bases, Tm 66
SSR size	237 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC58
Source Species	Triticum aestivum